Culture expansion of primary cells evokes highly reproducible DNA methylation (DNAm) changes at specific sites in the genome. These changes might be due to an directly regulated epigenetic process, or

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Isolation and long-term expansion of murine epidermal stem-like cells

表皮干细胞

细胞培养

Epidermis is the most outer layer of the skin and serves as a physical barrier protecting the internal tissues from mechanical and environmental insults. Its constant renewing and regeneration capacit

干细胞与再生医学数据中心2022-02-20 更新60

有/无葡萄糖培养的MDSC转录组芯片

转录组分析

细胞培养

纯化的CD34+细胞通过造血干细胞扩增培养基培养7-8天后，在有或者无葡萄糖的条件下进行G-CSF、GM-CSF刺激后，使用TRIZOL法提取总RNA。通过Agilent Bioanalyzer 2100检测RNA质量后，进一步用RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#7925

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Additional file 3 of The effect of medium supplementation and serial passaging on the transcriptome of human adipose-derived stromal cells expanded in vitro

细胞培养

基因表达

Gene ontology terms for ASCs serially expanded in FBS and pHPL. Complete list of enriched GO terms for ASCs serially expanded in pHPL or FBS (P0 â P5). This data relates to Table 1. (XLSX 1017 kb)

DataCite Commons2020-08-26 更新70

yagpm3_2

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