DNA Stable Isotope Probing Metagenomics of Biochar-Amended Soils

The dynamics of individual microbial populations and their gene functions in agricultural soils amended with biochar remain elusive but are important for a deeper understanding of nutrient cycling and carbon (C) sequestration. In this study, we coupled DNA stable isotope probing (SIP) with shotgun metagenomics in order to target the active community in microcosms which contained soil collected from biochar-amended and control plots under napiergrass cultivation.