Improving the recovery for dissolved eDNA State: A comparative analysis of isopropanol precipitation, magnetic bead extraction, and centrifugal dialysis

# Improving the Recovery for Dissolved eDNA State: A Comparative Analysis of Isopropanol Precipitation, Magnetic Bead Extraction, and Centrifugal Dialysis [https://doi.org/10.5061/dryad.z08kprrnk](https://doi.org/10.5061/dryad.z08kprrnk) ## Description of the data and file structure This dataset contains qPCR data from an experiment to evaluate methods for extracting eDNA from its dissolved state This dataset contains one excel file "dissolved state data.xlsx" with the following columns 1\) Position: This indicated the position on the qPCR plate 2\) ID: This indicated the sample identification. Please refer to the methods section for accurate interpretation of these values 3\) ct: This value indicated the ct value expired from the qPCR instrument. Black or NA cells indicate no qPCR amplification of DNA 4\) Probe: Indicated what qPCR probe was used for the given measurement 5\) Method: shows the method of extraction. N = None, M = magnetic bead, I = isopropanol precipitation A = Amicon centrifugal dialysis 6\) Inhibitor: Shows what inhibitor removal method was used. N = None, Z = Zymo ## Sharing/Access information There are no links to other publicly accessible locations of the data:# 提升溶解态环境DNA（environmental DNA, eDNA）回收率：异丙醇沉淀法、磁珠提取法与离心透析法的对比分析 [https://doi.org/10.5061/dryad.z08kprrnk](https://doi.org/10.5061/dryad.z08kprrnk) ## 数据与文件结构说明 本数据集包含一项用于评估溶解态环境DNA（eDNA）提取方法的实验所产生的定量聚合酶链反应（quantitative PCR, qPCR）数据。本数据集包含一个Excel文件"dissolved state data.xlsx"，其包含如下列项： 1. 孔位（Position）：表示定量聚合酶链反应（qPCR）板上的反应孔位置。 2. 样本编号（ID）：表示样本识别标识，相关数值的准确解读请参阅论文的方法学章节。 3. Ct值（ct）：该数值为qPCR仪器导出的循环阈值，单元格为黑色或标注NA则表示未检测到DNA的qPCR扩增信号。 4. 探针（Probe）：表示本次检测所使用的qPCR探针类型。 5. 提取方法（Method）：标注所用的eDNA提取方式，其中N代表无处理，M代表磁珠提取法，I代表异丙醇沉淀法，A代表Amicon离心透析法。 6. 抑制剂去除方法（Inhibitor）：标注所用的抑制剂去除处理方式，其中N代表无处理，Z代表Zymo试剂盒法。 ## 共享与访问信息 本数据集未提供其他公开可用数据的相关链接。