Transcriptome profiling in knock-in mouse models of Huntington's disease [Hippocampus_mRNA]

Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder that is characterized by motor, cognitive, and psychiatric alterations. The mutation responsible for this disease is an abnormally expanded and unstable CAG repeat within the coding region of the gene encoding huntingtin (Htt). Knock-in mouse models of HD with human exon 1 containing expanded CAG repeats inserted in the murine huntingtin gene (Hdh) provide a genetic reconstruction of the human causative mutation within the mouse model. The goal of this study is RNA expression profiling by RNA sequencing (RNA-seq) in 2, 6, and 10 month old knock-in mice with CAG lengths of 20, 80, 92, 111, 140, 175 along with littermate control wild-type animals mRNA expression profiles were obtained via RNA-seq analysis performed on tissue samples from the hippocampus of 2, 6, and 10 month old knock-in mice with CAG lengths of 20, 80, 92, 111, 140, 175 along with littermate control wild-type animals.亨廷顿舞蹈症（Huntington's disease, HD）是一类常染色体显性遗传的神经退行性疾病，以运动、认知及精神功能异常为核心临床特征。该病的致病突变位于亨廷顿蛋白（huntingtin, Htt）编码基因的编码区，为异常扩增且不稳定的CAG三核苷酸重复序列。将携带扩增CAG重复序列的人类外显子1插入小鼠亨廷顿基因（Hdh）所构建的HD敲入小鼠模型，可在小鼠体内重现人类致病突变的遗传背景。本研究旨在通过RNA测序（RNA-seq）开展RNA表达谱分析：实验对象涵盖2、6、10月龄且CAG重复长度分别为20、80、92、111、140、175的敲入小鼠，以及同窝野生型对照动物；最终通过对上述小鼠海马组织的RNA-seq分析，获取其mRNA表达谱。