Additional file 5: Figure S4. of Mycobacterium tuberculosis whole genome sequencing and protein structure modelling provides insights into anti-tuberculosis drug resistance

The relationship between the total number of non-synonymous SNPs in candidate loci and the MIC values. The size of the circle represents the number of isolates. a) Ethambutol (embB, embA, embA promoter, embC, embR and ubiA). b) Streptomycin (rpsL, rrs). The size of the circles is proportional to the frequency. The MIC values tend to increase with the number of non-synonymous mutations (ethambutol: rho = 0.24, slope = 0.29, p = 0.003; streptomycin: rho = 0.48, slope = 3.59, p = 1.65 × 10-8). The horizontal blue lines refer to the resistance cut-offs. (ZIP 92 kb)

候选位点中非同义单核苷酸多态性（single nucleotide polymorphism, SNP）总数与最低抑菌浓度（minimum inhibitory concentration, MIC）值之间存在关联。圆形的尺寸代表分离菌株的数量。a) 乙胺丁醇（ethambutol）相关位点：embB、embA、embA启动子、embC、embR及ubiA。b) 链霉素（streptomycin）相关位点：rpsL、rrs。圆形大小与对应频率呈正比关系。MIC值随非同义突变数量的增加呈升高趋势（乙胺丁醇：rho=0.24，斜率=0.29，p=0.003；链霉素：rho=0.48，斜率=3.59，p=1.65×10^-8）。图中水平蓝色参考线为耐药临界值。（压缩包大小92 kb）