Comparative population genetic analysis of bocaccio rockfish Sebastes paucispinis using anonymous and gene-associated simple sequence repeat loci

Comparative population genetic analyses of traditional and emergent molecular markers aid in determining appropriate use of new technologies. The bocaccio rockfish Sebastes paucispinis is a high-gene-flow marine species off the west coast of North America that experienced strong population decline over the past three decades. We used 18 anonymous and 13 gene associated simple sequence repeat loci (EST-SSRs) to characterize range-wide population structure with temporal replicates. No FST-outliers were detected using the LOSITAN program, suggesting that neither balancing nor divergent selection affected the loci surveyed. Consistent hierarchical structuring of populations by geography or year class was not detected regardless of marker class. The EST-SSRs were less variable than the anonymous SSRs, but no correlation between FST and variation or marker class was observed. General Linear Model analysis showed that low EST-SSR variation was attributable to low mean repeat number. Comparativ...传统与新兴分子标记的群体遗传学比较分析，可为新技术的合理应用提供科学依据。博卡乔岩鱼（Sebastes paucispinis）是北美西海岸海域的高基因流海洋物种，近三十年来种群数量经历了显著衰退。本研究采用18个匿名简单序列重复位点与13个基因关联型简单序列重复标记（表达序列标签-简单序列重复，EST-SSRs），结合时间重复采样策略，对该物种的全分布区群体结构进行解析。经LOSITAN程序检测，未发现群体分化系数（Fixation Index, FST）离群位点，提示本次分析涉及的所有位点均未受到平衡选择或歧化选择的影响。无论使用哪类分子标记，均未检测到基于地理分区或同龄组的一致性分层群体结构。EST-SSRs的多态性水平低于匿名SSR，但未观察到FST值与多态性水平或标记类型之间存在显著相关性。广义线性模型（General Linear Model, GLM）分析结果显示，EST-SSRs较低的多态性可归因于其平均重复数偏低。比较...