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RT-PCR validation of Nanostring hits.
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创建时间：
2025-01-01
相关数据集
Pseudogenes as Weaknesses of ACTB (Actb) and GAPDH (Gapdh) Used as Reference Genes in Reverse Transcription and Polymerase Chain Reactions
The genes encoding β-actin (ACTB in human or Actb in mouse) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH in human or Gapdh in mouse) are the two most commonly used references for sample normali
NIAID Data Ecosystem40
Validation of the gene interactions in the T-cell activation network.
Gene expression levels of target genes were assessed by real time PCR in PBMCs from 16 new healthy controls after stimulation with IL10, IL4 or CTLA4 (stimulus) for 12 to 24h. Results are described as
Figshare2015-12-02 更新40
Top 20 overexpressed genes (>10 fold) in both CS-1/ER and CS-1/PR resistant cell lines.
*Genes in bold were chosen for real-time RT-PCR validation.
Figshare2015-12-02 更新20
PCR conditions for amplification of exon 5 of STAT6, off-target regions, and quantitative RT-PCR 96 wells plates.
PCR conditions for amplification of exon 5 of STAT6, off-target regions, and quantitative RT-PCR 96 wells plates.
NIAID Data Ecosystem40
Quantitative PCR (RT-qPCR) validation of RNA-seq using liver samples from each of the four comparisons, illustrated in Fig 1.
Quantitative PCR (RT-qPCR) validation of RNA-seq using liver samples from each of the four comparisons, illustrated in Fig 1.
NIAID Data Ecosystem20
