Dietary induced epigenetic reprogram in mouse intestinal enterocytes

Bl6 mice were fed with New Western diet 1 (NWD1) or Americal Instititue of Nutrition 76A (AIN76A or AIN) diet from weaning for 3 months. Single intestinal epithelial cells were FACS isolated by the Epcam+, CD45- , and subject to scACTAseq assay. Overall design: Examine roles of dedifferentiation of Bmi1 cell in regeneration of intestinal stem cells and its impact on mucosal homeostasis under normal physiogical condition (fed with various diets).