CRISPR off-target sequencing analysis

Exome sequencing on two independent models of myeloid neoplasms was performed to query off-target effects of Cas9 nuclease. Aligned sequencing data was filtered for reads overlapping predicted off-target cut sites (as determined by algorithm by Hsu et al., 2013) and 161 hematopoiesis and leukemia genes recurrently mutated in hematologic malignancies. Bam files of the filtered sequencing reads for each sample, as well as all of the annotations required for filtering is included.

本研究针对两种独立的髓系肿瘤模型实施外显子组测序（Exome sequencing），旨在探究Cas9核酸酶（Cas9 nuclease）的脱靶效应。对已比对完成的测序数据进行过滤，筛选出与Hsu等学者2013年提出的算法所预测的脱靶切割位点重叠，且映射至161个在血液系统恶性肿瘤中频发突变的造血与白血病相关基因的测序读段（reads）。本数据集包含所有样本的过滤后测序读段的BAM文件（Bam files），以及过滤所需的全部注释信息。