Somatic Cell Fate Maintenance in Mouse Fetal Testes via Autocrine/Paracrine Regulation of anti-Mullerian hormone and Activin B

Fate determination and maintenance of fetal testes in most mammals occur cell autonomously as a result of the action of key transcription factors in Sertoli cell. In the mouse testis AMH and Activin B are required for the maintenance of Sertoli cell fate. In the absence of both AMH and Activin B (in the global dKO) Sertoli cells at the testis poles transdifferentiate into granulosa cells by embryonic day 15.5. Control testis (Amh+/+; Inhbb+/+), Control ovaries (Amh+/+; Inhbb+/+) and dKO (Amh-/-; Inhbb-/-) testis at E15.5多数哺乳动物胎儿睾丸的命运决定与维持以细胞自主方式进行，其分子机制为支持细胞（Sertoli cell）内关键转录因子的调控作用。在小鼠睾丸中，抗缪勒管激素（AMH）与激活素B（Activin B）是维持支持细胞命运所必需的因子。在同时缺失AMH与Activin B的全局双基因敲除模型（global dKO）中，胚胎发育至第15.5天（E15.5）时，睾丸两极的支持细胞会转分化为颗粒细胞。本数据集涵盖E15.5时期的三类样本：野生型睾丸（Amh+/+; Inhbb+/+）、野生型卵巢（Amh+/+; Inhbb+/+）以及双基因敲除睾丸（Amh-/-; Inhbb-/-）。