We examined the off-targets of new base editing tools at RNA level Overall design: cells transfected with different base editing tools or GFP were cultured for 48 hours and total RNA was purified for
some factors which may influence the activity of base editing were chose to carry out the experiment.and the data of this experiment was acquired from GENEWIZ and analyzed by BE-Analyzer.
The ability to engineer proteins co-opted from nature to suit the needs of researchers is pivotal to a future, pragmatic biology. For example, CRISPR proteins adapted from bacterial immune systems are
Increasing the specificity of base editing with engineered Cas9-APOBEC3B deaminase fusions, the related data including rice reference sequence, rice plants whole-genome sequences and targets replicon
