Numeric data from: Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells

Stem and progenitor cell mitosis is essential for tissue development and homeostasis. How these cells ensure proper chromosome segregation, and thereby maintain mitotic fidelity, in the complex physiological environment of a living animal is poorly understood. Here we use in situ live-cell imaging of C. elegans germline stem and progenitor cells (GSPCs) to ask how the signaling environment influences stem and progenitor cell mitosis in vivo. Through a candidate screen we identify a new role for the insulin/IGF receptor (IGFR), daf-2, during GSPC mitosis. Mitosis is delayed in daf-2/IGFR mutants, and these delays require canonical, DAF-2/IGFR to DAF-16/FoxO insulin signaling, here acting cell non-autonomously from the soma. Interestingly, mitotic delays in daf-2/IGFR mutants depend on the spindle assembly checkpoint but are not accompanied by a loss of mitotic fidelity. Correspondingly, we show that caloric restriction, which delays GSPC mitosis and compromises mitotic fidelity, does not..., This dataset contains all numeric data reported in the paper \"Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells\". Details on data collection and processing can be found in the methods section of our paper. Measurement data include the duration of mitosis in germline stem cells in a range of genetic backgrounds and/or experimental conditions, the number of nuclei and mitotic cells per germline proliferative zone and germ line and germline stem cell fluorescent intensity measurements., , # Numeric data for manuscript: Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells [https://doi.org/10.5061/dryad.sn02v6xfp](https://doi.org/10.5061/dryad.sn02v6xfp) ## Description of the data and file structure These data were collected in support of the paper  \"Non-autonomous insulin signaling delays mitotic progression in *C. elegans *germline stem and progenitor cells which is available on bioRxiv ([https://doi.org/10.1101/2024.06.28.601188](https://doi.org/10.1101/2024.06.28.601188)) and in press at PLoS Genetics. ### Files and variables #### File: Numeric\_Data\_for\_all\_figure\_panels.zip **Description:** Data are organized by figure, with one folder per figure (e.g. Figure_1_RawData). Each folder contains one or more CSV files, with the file name indicating to which figure panel the numeric data belong. CSV files were generated by exporting data tables from Matlab. The first row gives the column name. Missing val...干细胞和祖细胞的有丝分裂对于组织发育与稳态维持至关重要。然而，在活体动物的复杂生理环境中，这些细胞如何确保染色体正确分离、进而维持有丝分裂保真度，目前仍知之甚少。 本研究通过对秀丽隐杆线虫（C. elegans）生殖系干细胞和祖细胞（germline stem and progenitor cells, GSPCs）进行原位活细胞成像，探究信号微环境如何在体内调控干细胞与祖细胞的有丝分裂过程。通过候选基因筛选，我们发现胰岛素/胰岛素样生长因子受体（insulin/IGF receptor, IGFR）daf-2在GSPCs有丝分裂中具有全新功能。daf-2/IGFR突变体的有丝分裂进程出现延迟，且该延迟依赖于经典的DAF-2/IGFR至DAF-16/FoxO胰岛素信号通路，且该作用由体细胞非细胞自主性介导。有趣的是，daf-2/IGFR突变体的有丝分裂延迟依赖于纺锤体组装检验点，但并未伴随有丝分裂保真度的丧失。进一步研究表明，能够延迟GSPCs有丝分裂并损害有丝分裂保真度的热量限制，并未[...]。 本数据集包含论文《Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells》中报道的全部数值型数据。数据采集与处理的详细步骤可参见本文的方法部分。本数据集的测量数据包括：不同遗传背景及/或实验条件下生殖系干细胞的有丝分裂时长、每个生殖系增殖区与生殖腺内的细胞核及有丝分裂细胞数量，以及生殖系干细胞的荧光强度测量值。 # 论文数值型数据：Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells [https://doi.org/10.5061/dryad.sn02v6xfp] ## 数据与文件结构说明 本数据集为支持论文《Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells》而收集，该论文已发布于bioRxiv（[https://doi.org/10.1101/2024.06.28.601188]）并被《PLoS Genetics》接收待刊。 ### 文件与变量 #### 文件：Numeric_Data_for_all_figure_panels.zip **说明：** 数据按图片进行组织，每个图片对应一个文件夹（例如Figure_1_RawData）。每个文件夹内包含一个或多个CSV文件，文件名标注了该数值数据对应的图片面板。 CSV文件通过从Matlab中导出数据表格生成，第一行为列名。缺失值[...]