Parameter estimates and their uncertainties.

Parameter estimates and local approximations of their uncertainty after the traditional, the first optimized, and the second optimized experiment. Shown are the system parameters which are believed to be identical from cell to cell: the degradation rate of , the dissociation rate for the dissociation of Akt from the plasma membrane, the inhibitor constant for LY29, and the association rates for the binding of iRap to the FKBP domain of CF-p85, for the formation of the trimeric complex of CF-p85, iRap, and Lyn-FRB, and for the recruitment of Akt to the plasma membrane. and denote the parameter estimate and its corresponding uncertainty after the experiment was performed.