Inventory of High-resolution phylogenetic profiles of the planktonic microbial communities (via 16S and 18S rRNA gene amplicons) from Shark River Slough and Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, 2017 - ongoing
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Planktonic microbial communities mediate many vital biogeochemical processes in wetland ecosystems, yet compared to other aquatic ecosystems, like oceans, lakes, rivers, or estuaries, they remain relatively underexplored. Our study site, the Florida Everglades (USA)—a vast iconic wetland consisting of a slow-moving system of shallow rivers connecting freshwater marshes with coastal mangrove forests and seagrass meadows—is a highly threatened model ecosystem for studying salinity and nutrient gradients, as well as the effects of sea level rise and saltwater intrusion. This dataset provides the first high-resolution phylogenetic profiles of planktonic bacterial and eukaryotic microbial communities (using 16S and 18S rRNA gene amplicons) from these environments. The dataset contains 16S and 18S rRNA data from 2017, and contains 16S rRNA data for monthly (2019) and quarterly water samples (2020-ongoing). The 2017 data are published in Laas et al. 2022. A detailed list of sequence data and their accession numbers in GenBank is provided and will be updated as more data are published. This data package is an inventory of sequence read archive (SRA) entries available through GenBank BioProject PRJNA525456 (at https://www.ncbi.nlm.nih.gov/bioproject/PRJNA525456) and BioProject PRJNA1018945 (at https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1018945). This data package is associated with the following publication: Laas, P., Ugarelli, K., Travieso, R., Stumpf, S., Gaiser, E. E., Kominoski, J. S., & Stingl, U. (2022). Water column microbial communities vary along salinity gradients in the Florida Coastal Everglades wetlands. Microorganisms, 10(2), 215. https://doi.org/10.3390/microorganisms10020215 Instead of citing this package, which is an inventory, please cite the original GenBank data or journal article, as appropriate. Citation guidance for the journal article is available on the respective publisher's website.
浮游微生物群落介导着湿地生态系统中诸多关键生物地球化学过程,但相较于海洋、湖泊、河流或河口等其他水生生态系统,其研究仍相对不足。 本研究的采样地点为美国佛罗里达大沼泽地——这是一片广阔的标志性湿地,由连接淡水沼泽与沿海红树林、海草床的缓流浅河水系构成,是用于研究盐度与营养梯度、海平面上升及海水入侵效应的高度受威胁的模式生态系统。 本数据集首次提供了此类生境中浮游细菌与真核微生物群落的高分辨率系统发育谱(采用16S和18S rRNA基因扩增子测序技术)。数据集包含2017年的16S与18S rRNA测序数据,以及2019年月度水样和2020年至今季度水样的16S rRNA测序数据。其中2017年的数据已发表于Laas等人2022年的研究中。本数据集还提供了序列数据及其在基因银行(GenBank)中的登录号详细列表,并将随着更多数据发表持续更新。 本数据包为通过基因银行(GenBank)生物项目PRJNA525456(网址:https://www.ncbi.nlm.nih.gov/bioproject/PRJNA525456)与PRJNA1018945(网址:https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1018945)可获取的序列读取存档(Sequence Read Archive, SRA)条目清单。本数据包关联以下已发表学术论文: Laas, P., Ugarelli, K., Travieso, R., Stumpf, S., Gaiser, E. E., Kominoski, J. S., & Stingl, U. (2022). 佛罗里达沿海大沼泽地湿地中水柱微生物群落沿盐度梯度发生变化. Microorganisms, 10(2), 215. https://doi.org/10.3390/microorganisms10020215 请注意,本数据包仅为序列条目清单,请勿直接引用本数据包,请根据实际情况引用原始GenBank数据或期刊论文。期刊论文的引用规范可在对应出版商的官方网站查询。



