GNPS Germ-Free Mice Inoculated with or without Lactobacillus rhamnosus under Tryptophan-Sufficient/Deficient Diet

Lacticaseibacillus rhamnosus (LGG), a promising probiotic for gastrointestinal disorders, has been found to significantly impact the host's intestinal metabolome, excluding pathobiont bacteria colonization. However, the specific identity and health impact of LGG-dependent metabolites remain poorly understood. This study aims to investigate the host-LGG-tryptophan interaction by analyzing feces and serum from LGG mono-associated GF mice fed trp-sufficient or trp-free diets.