Functional profiling and visualization of the sphingolipid metabolic network in vivo

This dataset contains a comprehensive collection of z-stack confocal images from whole-mount adult Drosophila melanogaster brains. The images document the detailed cell-type expression patterns of 52 genes essential for sphingolipid metabolism. The expression of each gene was visualized using the GAL4-UAS binary system and immunostainings. Specific GAL4 reporter lines (C-terminus 3XHA-T2A-GAL4 insertion), which faithfully recapitulate the endogenous expression of the target genes, were used to drive a nuclear red fluorescent protein (UAS-mCherry-nls). Anti-Elav and anti-Repo stainings were used to label neurons and glia, respectively. Anti-HA stainings were used to visualize protein distributions.本数据集包含一套完整的成年黑腹果蝇（Drosophila melanogaster）全脑整装标本的共聚焦z-stack图像集合。该图像集记录了52个鞘脂代谢必需基因的详细细胞类型表达模式。各靶基因的表达均通过GAL4-UAS双元表达系统与免疫染色技术完成可视化。研究使用可精准重现靶基因内源表达模式的特定GAL4报告品系（C端3XHA-T2A-GAL4插入型），以驱动核红色荧光蛋白（UAS-mCherry-nls）的表达。分别采用抗Elav抗体染色与抗Repo抗体染色标记神经元与神经胶质细胞，抗HA抗体染色则用于可视化蛋白的分布情况。