Screening_Zebrafish_for_CRISPR_induced_indels

NIAID Data Ecosystem2026-03-12 收录

CRISPR基因编辑效应

斑马鱼突变检测

数据链接：

https://www.ncbi.nlm.nih.gov/sra/ERP006540 数据链接链接失效反馈

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资源简介：

Loci from CRISPR injected Zebrafish was amplified and sequenced to identify indels induced by the CRISPR/Cas9 system.

创建时间：

2021-02-04

Mus musculus strain:B6.129S-Atoh1-GFP Targeted Locus (Loci)

CRISPR基因编辑效应

耳蜗基因编辑

To evaluate the DNA cleavage efficiency of Cas9 delivered as protein complexed with either EMX sgRNA or GFP sgRNA in liposomes to the mouse cochlea in vivo, we measured the modification of on-target g

NIAID Data Ecosystem70

sgRNAs used in this study and CRISPR targeting efficiency measured by TIDE analysis.

CRISPR基因编辑效应

INDEL突变检测

Genomic DNA was harvested from CRISPR-targeted and non-targeted cells and the targeted locus was amplified by PCR (~200bp upstream and downstream of the predicted cut site), and sequenced via capillar

NIAID Data Ecosystem40

Genome-wide maps of chromatin state in human primary CAR-T cells [ChIPseq, ATAC-seq]

CAR-T细胞工程

CRISPR基因编辑效应

We report the transcriptomic and epigentic profile of human primary CD19-28z CAR-T cells. Overall design: CRISPR gene editing was used to delete MED12 or the "safe harbor" control gene AAVS1. Gene-edi

NIAID Data Ecosystem40

Proteome of long-term expanded human CD4+ and CD8+ T cells

细胞蛋白质组学

CRISPR基因编辑效应

We analyzed the total proteome of CD4+ and CD8+ T cells isolated from human peripheral blood mononuclear cells (PBMC), and cultured to perform a CRISPR/CAS9 edition of their genome, in order to introd

NIAID Data Ecosystem70

Deep sequencing of the edited genomic sites after targeted knock-in (exogenous EGFP integrated into the genome)

CRISPR基因编辑效应

靶向敲入indel分析

This study was performed to compare the frequencies of indel formation at the edited genomic sites (on-target sites) upon targeted knock-in using CRISPR/Cas9 nickases and those elicited by the use of

NIAID Data Ecosystem40

Screening_Zebrafish_for_CRISPR_induced_indels

资源简介：

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