RNA-seq analysis for zebrafish tumors driven by overexpression of human IRF4

RNA-seq analysis was performed using zebrafish primary tumors and control thymus to analyze gene expression changes after IRF4 overexpression. Human IRF4 gene was overexpressed under the zebrafish lck promoter. Tumor cells were harvested from IRF4-transgenic zebrafish (lck-IRF4) with or without heterozygous p53 mutation. Normal thymus cells were also harvested from control zebrafish (lck-mCherry).