Homo sapiens Genome sequencing

To increase the targeting scope and diversify the editing patterns of base editors, we designed a subset of base editors derived from the Cas9 ortholog from Staphylococcus aureus (SaCas9) in which cytosine or adenosine deaminase was internally inlaid into various locations of the SaCas9 protein. The resulting base editors were characterized with a set of endogenous genomic sites in HEK293T cells and were found to have distinct editing features to the N-terminal linked SaCas9 CBE variants.