1. Despite widespread recognition of its great promise to aid decision-making in environmental management, the applied use of metabarcoding requires improvements to reduce the multiple errors that
Metabarcoding of fungi from 20 lakes in North-East Germany. Water and sediment samples were taken near the shore and in the open water. Different size rDNA amplicons were generated and sequenced with
A custom mcrA gene (Methyl-coenzyme M reductase A) reference database with 39,544 sequences and assigned taxonomy for studying methanogens with metabarcoding. Sequences in database were extracted from
Here, we leverage metabarcoding in 190 soil microbiome samples (7,600 subsamples), covering all major biomes and soil types, to generate a global view of taxonomic and functional diversity in two comm
