Genome-wide CRISPR screening using cell lines that harbor the mutant hTERT-CRISPR allele.

To assess the biological significances of p-hTERT in ALT cells, we used the CRISPR-Cas9 system targeting the hTERT gene and performed genome-wide CRISPR screening, which identified genes in the Fanconi anemia/BRCA pathway as synthetic lethal partners of hTERT. We established two U2OS cell clones with heterozygous mutations in the hTERT gene locus by CRISPR-Cas9 system. Genome-scale CRISPR loss of function screen were performed using these cells. Two replicates for each cell were included.