Kelp growth data from seasonal global change experiments examining the effects of seasonal variation in light availability and nutrients on the response of three high-latitude kelp species

This kelp growth dataset results from two, month-long global change laboratory experiments conducted in Sitka, Alaska from February 12 to March 18, 2020 (\"winter\" experiment) and August 15 to September 16, 2020 (\"summer\" experiment). These experiments were used to tease apart the effects of seasonal variation in light availability and nutrients on the response of three high-latitude kelp species (Macrocystis pyrifera, Hedophyllum nigripes, and Neoagarum fimbriatum) to pH and temperature. Experimental controls were designed to approximate current environmental conditions in Sitka Sound, and treatments were based on projected end-of-century scenarios of ocean acidification (OA) and warming (OW) for this region. At the end of the experiments, the investigators assessed the seasonal impact of OW and OA on kelp growth rates, thallus nitrogen content, and carbon acquisition strategy based on thallus δ13C values. Finally, to test whether kelp palatability was impacted by future warming and acidification, tissue of H. nigripes and N. fimbriatum grown during the experiments was used to perform feeding assays with a common kelp forest consumer. These experiments were performed to improve our understanding of how global change will alter marine primary producer resources by integrating natural variation in environmental drivers. This project was overseen by Dr. Kristy Kroeker and her Ph.D. student Lauren Bell, both at the University of California Santa Cruz. Experiments took place in the basement laboratory at the Sitka Sound Science Center in Sitka, AK. This work was supported by the National Science Foundation (OCE-1752600), the David and Lucile Packard Foundation, the North Pacific Research Board's Graduate Student Research Award (1748-02), the UCSC Physical and Biological Sciences 2019 Future Leaders in Coastal Science Award, the Seymour Marine Discovery Center's Student Research and Education Award, and the California State University Monterey Bay Undergraduate Research Opportunities Center.本大型海藻生长数据集源自两次为期一个月的全球变化实验室操控实验，实验于2020年2月12日至3月18日（“冬季实验”）、2020年8月15日至9月16日（“夏季实验”）在阿拉斯加州锡特卡开展。本实验旨在厘清光照可获得性与营养盐的季节变化对三种高纬度大型海藻——巨藻（Macrocystis pyrifera）、黑叶藻（Hedophyllum nigripes）以及新琼脂藻（Neoagarum fimbriatum）——响应海水pH与温度的调控效应。实验对照组的设置旨在近似锡特卡湾当前的环境条件，实验组则依据该区域海洋酸化（Ocean Acidification, OA）与海洋变暖（Ocean Warming, OW）的世纪末预测情景设计。实验结束后，研究者评估了海洋变暖和酸化对大型海藻生长速率、叶状体氮含量以及基于叶状体碳同位素δ¹³C值的碳获取策略的季节影响。此外，为验证未来海洋变暖和酸化是否会影响大型海藻的适口性，研究者利用实验中培育的黑叶藻与新琼脂藻的组织，开展了针对常见大型海藻林消费者的摄食实验。 本实验通过整合环境驱动因子的自然变异，旨在加深我们对全球变化如何改变海洋初级生产者资源的认知。本项目由加州大学圣克鲁兹分校的克里斯蒂·克罗克（Kristy Kroeker）博士及其博士生劳伦·贝尔（Lauren Bell）主导。实验在阿拉斯加州锡特卡的锡特卡湾科学中心地下实验室完成。本研究得到了美国国家科学基金会（National Science Foundation, OCE-1752600）、大卫与露西尔·帕卡德基金会、北太平洋研究委员会研究生研究奖（1748-02）、加州大学圣克鲁兹分校物理与生物科学学院2019年海岸科学未来领袖奖、西摩海洋探索中心学生研究与教育奖以及加州州立大学蒙特雷湾分校本科生研究机会中心的资助。