UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity

To better understand the impact of Ufl1 expression in T cells on regulating tumor microenvironment dynamics, we performed single-cell RNA sequencing on purified CD45+ immune cells from MC38 xenografts harvested from Ufl1 cKO or WT C57BL/6 mice. MC38 tumors were established by subcutaneously injecting 1×10^5 MC38 cells into 8-week-old WT and Ufl1 cKO mice, CD45+ immune cells were sorted by FACSAria III (BD) from subcutaneous MC38 tumors for scRNA-seq analysis.