In this project, we performed directed evolution on an E. coli Nissle 1917 strain to increase the yields of extracellular protein through the curli secretion pathway. We performed random mutagenesis o
Recombinant proteins are ubiquitously applied in fields like research, pharma, diagnostics or the chemical industry. To provide the full range of useful proteins, novel expression hosts need to be est
Comparison tables for iJV1220. Tables comparing the results obtained using the iJV1220 model for the protein secreting strains with those of the plasmid bearing and wild-type strains. (XLS 11751 kb)
Bacteriocin release proteins (BRPs) can be used for the release of heterologous proteins from the Escherichia coli periplasm into the culture medium. However, high-level expression of BRP causes appar
aStrain names indicate promoter used. bDetermined by qPCR using an absolute quantification of transcript copy number. cDetermined by RT-qPCR using an absolute quantification of transcript copy number
