A high-throughput skim-sequencing approach for genotyping, dosage estimation and identifying translocations

An optimized, high-throughput and cost-effective genotyping method applicable to various crop breeding populations is very important in this genomic era. We have developed an optimized Nextera skim-sequencing (skim-seq) approach to genotype different populations that can be used for genetics studies and genomics-assisted breeding. We performed skim-seq on a variety of populations developed through doubled haploid (DH) technology, inter-specific recombinants developed through introgression, amphidiploid developed through wide crosses, and on known monosomic samples. 1. A doubled haploid (DH) population consisting of 48 lines from the cross of spring wheat (Triticum aestivum) cultivars CDC Stanley and CDC Landmark developed by the Crop Development Centre at the University of Saskatchewan. We genotyped these DH with skim-seq and identified the genomic segments contributed by each of the two parental lines. 2. A population of 335 back cross generation 1 (BC1) skim-seq samples for whea...在当前基因组学时代，一种适用于多种作物育种群体的优化、高通量且经济高效的基因分型方法极为关键。我们开发了一种优化的Nextera skim-sequencing（skim-seq）方法，用于对不同群体开展基因分型工作，该方法可应用于遗传学研究及基因组辅助育种。我们对多种群体进行了skim-seq测序，包括通过双单倍体（doubled haploid, DH）技术培育的群体、通过渐渗法获得的种间重组体、通过远缘杂交培育的双二倍体，以及已知的单体系样本。 1. 由萨斯喀彻温大学作物开发中心培育的、来源于春小麦（*Triticum aestivum*）品种CDC Stanley与CDC Landmark杂交的48份株系双单倍体（DH）群体。我们通过skim-seq对该DH群体进行基因分型，并鉴定出了两个亲本各自贡献的基因组片段。 2. 包含335份小麦回交一代（back cross generation 1, BC1）skim-seq样本的群体，其相关用途原文未完整给出