Identification of the altered transcripts in the NMF291-/- cerebellum by exon array

The U2 snRNA is a basal component of the major spliceosome, which is responsible for >90% human pre-mRNA splicing. A 5-nucleotide deletion in one of the mouse U2 snRNA genes (Rnu2-8) causes cerebellar granule cell degeneration in the NMF291 mouse mutant strain. To identify the altered transcripts in the NMF291-/- cerebellum, we interograted Affy. mouse 1.0 ST Exon arrays with total RNAs from three postnatal-30-day (P30) wild type and three NMF291-/- cerebella. Affy. mouse 1.0 ST Exon arrays were hybridized with total RNAs derived from three P30 female wild type and NMF291-/- mice (biological replicates).U2小核RNA（U2 snRNA）是主要剪接体（major spliceosome）的基础组成成分，该剪接体负责人类90%以上的前体mRNA（pre-mRNA）剪接过程。小鼠U2 snRNA基因（Rnu2-8）中的一处5核苷酸缺失突变，可导致NMF291突变小鼠品系出现小脑颗粒细胞变性。为鉴定NMF291-/-小鼠小脑内的差异转录本，我们采用Affymetrix（简称Affy.）小鼠1.0 ST外显子芯片（Exon arrays），对3只出生后30天（P30）的雌性野生型小鼠与3只同基因型NMF291-/-小鼠的小脑总RNA进行芯片杂交分析，两组样本均设置3个生物学重复。