We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 in the protospacer of genomes in both protoplasts and regenerated ric
We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 in the protospacer of genomes in both protoplasts and regenerated ric
The goal of this study is to assess T-DNA free base editing in poplar. Using Agrobacterium-mediated transformation, poplar callus was transformed with a base editing construct. Three base-edited lines