DNA methylation in human pancreatic cancer vs. adjacent normal pancreatic tissue. Homo sapiens

HumanMethylation 450K microarrays were used to identify aberrant DNA methylation in pancreatic adenocarcinoma (n=14), juice-circulating cancer cells (n=4), cancer associated fibroblasts (n=3), against adjacent normal pancreatic tissues (n=7) Bisulphite converted DNA from the 28 samples were hybridised to the Illumina HumanMethylation450 Beadchip v1.2 Overall design: Pancreatic tumor samples (n=14), pancreatic juice circulating cancer cells (n=4), cancer associated fibroblasts (n=3) and adjacent normal pancreatic tissues (n=7)