C1 single-cell RNA-seq of Adult Human spermatoognia

To reveal distinct transcriptomes associated with spermatogonial stem cell renewal vs. initiation of differentiation, single-cell transcriptomes from Adult Human spermatogonia were subdivided into subpopulations based on the levels of ID4 mRNA (determined in this experiment). This correlates with distinct fates of corresponding mouse spermatogonia when assayed by transplantation, with ID4-EGFPbright cells highly enriched for SSCs, and ID4-EGFPdim cells enriched for progenitors. We used the Fluidigm C1 instrument to capture individual spermatogonia for SMART-Seq2 single-cell RNA-seq.为阐明精原干细胞（spermatogonial stem cell）自我更新与分化启动各自关联的独特转录组特征，本研究基于本次实验测定的ID4信使RNA（ID4 mRNA）表达水平，将人类成体精原细胞的单细胞转录组数据划分为多个亚群。该分类结果与经移植实验验证的对应小鼠精原细胞的独特细胞命运高度吻合：ID4-EGFP强阳性细胞显著富集精原干细胞，ID4-EGFP弱阳性细胞则富集于精原祖细胞群体。本研究采用Fluidigm C1（Fluidigm C1）仪器捕获单个精原细胞，通过SMART-Seq2技术开展单细胞RNA测序（RNA-seq）。

搜集汇总

背景与挑战

背景概述

该数据集是一个人类成年精原细胞的单细胞RNA测序数据集，包含635个样本，总数据量达193.34 GB，使用Fluidigm C1仪器和SMART-Seq2方法进行测序。其核心目的是通过分析ID4 mRNA水平来区分精原干细胞更新与分化起始相关的转录组差异，数据来源于公开数据库GSE108977，适用于干细胞生物学和生殖医学研究。