Dynamics of calcineurin-regulated gene expression in S. cerevisiae

Gene expression was quantified in crz1 delete S. cerevisae cells over a time course following calcineurin activation by CaCl2. Cells were pretreated with buffer or the calcineurin inhibitor FK506 to allow the identification of calcineurin-regulated genes. A strain with a calcineurin-resistant mutation in the transcription factor Hcm1 was also examined. We show that calcineurin activation leads to downregulation of a many clusters of cell cycle-regulated genes, including those expressed at the G1/S and G2/M transitions.本研究对经氯化钙（CaCl₂）激活钙调磷酸酶（calcineurin）后的crz1缺失酿酒酵母（S. cerevisae）细胞开展了时序性基因表达定量分析。实验设置了缓冲液预处理组与钙调磷酸酶抑制剂FK506预处理组，以实现钙调磷酸酶调控基因的筛选鉴定；同时还针对转录因子Hcm1携带钙调磷酸酶抗性突变的酵母菌株进行了检测。本研究证实，钙调磷酸酶激活可引发多组细胞周期调控基因簇的表达下调，其中涵盖了在G1/S期与G2/M期转换阶段表达的基因。