A study of Neutrophils orchestrate IEL activation and intestinal homeostasis through microbial metabolite dimethyl fumarate. Huimin Chen et al.

The full-length GSDMD and GSDMD-N were determined by Western blotting in TCRγδ+CD8αα+ IELs, IECs, and the colon tissues (LB) of DSS-treated Cd177-/- and WT mice with/without DMF administration. See Figure 6H in the manuscript.采用蛋白质印迹法（Western blotting）检测了经葡聚糖硫酸钠（DSS）处理、伴或不伴富马酸二甲酯（DMF）给药的Cd177基因敲除（Cd177-/-）与野生型（WT）小鼠的TCRγδ+CD8αα+肠上皮内淋巴细胞（intraepithelial lymphocytes, IELs）、肠上皮细胞（intestinal epithelial cells, IECs）以及结肠组织（LB）中的全长GSDMD（Gasdermin D）与GSDMD-N蛋白表达情况。详见该手稿中的图6H。