Benchmarking single-cell RNA sequencing methods for the Human Cell Atlas

This cryopreserved HCA reference sample represents the basis for a systematic comparison of scRNAseq techniques. Overall design: The sample consists of two complex tissues (human PBMC and mouse colon, 80% of the sample content) and three cell lines (10% HEK293-RFP, 10% NIH3T3-GFP, <1% MDCK-Turbo650). The sample preparation aims to be standardized for all methods to allow a comparison of library preparation performance of the sample content.