Sugarcane stalk borer larvae were grown on artificial diet and maintained at 25°C and 60±10% relative humidity with a 14 h/10 h light/dark cycle. Second instar larvae were maintained under fasting con
PacBio platform were used to sequencing transcriptome of Bradysia odoriphaga and produce a single-molecule long-read dataset. The isoforms after polish and removed redundancy were then annotated by BL