The relative expression of four osteogenic genes under the different treatments.
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1) no ATRA, no BMP-2/7; 2) 1 µM ATRA, no BMP-2/7; 3) 1 µM ATRA, 5 ng/ml BMP-2/7; 4) 1 µM ATRA, 50 ng/ml BMP-2/7; 5) no ATRA, 5 ng/ml BMP-2/7; 6) no ATRA, 50 ng/ml BMP-2/7 for 1 day, 4 days and 7 days. (A) Runx2; (B) Collagen I; (C) Alkaline phosphatase; (D) Osteocalcin. The gene expression was first normalized to the corresponding β-actin gene expression for each sample. Then all the gene data were normalized to the gene data in control group on the 1st day. All data are presented as mean values together with the standard deviation (SD). *: ppp
1) 无全反式维甲酸(ATRA)及骨形态发生蛋白2/7(BMP-2/7)；2) 1 μM全反式维甲酸(ATRA)，无骨形态发生蛋白2/7(BMP-2/7)；3) 1 μM全反式维甲酸(ATRA)，5 ng/ml骨形态发生蛋白2/7(BMP-2/7)；4) 1 μM全反式维甲酸(ATRA)，50 ng/ml骨形态发生蛋白2/7(BMP-2/7)；5) 无全反式维甲酸(ATRA)，5 ng/ml骨形态发生蛋白2/7(BMP-2/7)；6) 无全反式维甲酸(ATRA)，以50 ng/ml骨形态发生蛋白2/7(BMP-2/7)分别处理1天、4天及7天。(A) Runt相关转录因子2(Runx2)；(B) I型胶原(Collagen I)；(C) 碱性磷酸酶(Alkaline phosphatase)；(D) 骨钙素(Osteocalcin)。首先将每个样本的基因表达量归一化至对应的β-肌动蛋白(β-actin)基因表达水平，随后将所有基因表达数据归一化至第1天对照组的基因表达数据。所有数据均以平均值±标准差(SD)的形式呈现。*: ppp
