Thermodynamic parameters associated with the thermal unfolding of RNase-A, Lysozyme and α-LA at pH 7.0.a, b, c.
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aErrors in ΔGDo, ΔHm and Tm from triplicate measurements are 5–9%, 2–5% and 0.1–0.6%, respectively.bValues in the parentheses are from [θ]222 measurements.cv, w, x and y represent concentrations of the osmolyte predicted from results given in Table 2 (see text). For instance, values of y for sorbitol are 1.20, 1.60 and 1.00 for RNase-A, lysozyme and α-LA, respectively.
a 三次重复测定所得的ΔGDo、ΔHm及Tm的误差分别为5%~9%、2%~5%与0.1%~0.6%。 b 括号内的数值源自222 nm处的椭圆率([θ]222)测量结果。 c v、w、x与y分别代表依据表2给出的结果(详见正文)预测得到的渗透调节剂(osmolyte)浓度。举例而言,针对山梨醇(sorbitol),其对应核糖核酸酶A(RNase-A)、溶菌酶及α-乳清蛋白(α-LA)的y值依次为1.20、1.60与1.00。
创建时间:
2015-12-02



