Supplementary Tables S1-S14 from Mutant NPM1 Directly Regulates Oncogenic Transcription in Acute Myeloid Leukemia

Table S1. Read normalized ratios of ChIPseq signal of untreated versus dTAG-13 treated OCI-AML3-NPM1c-FKBP12 cells.Table S2. Summary of NPM1c and NPM1 Chromatin Binding Targets.Table S3. Patient info for patient derived xenograft (PDX) models used.Table S4. ChIPseq reads per kilobase (rpk) data summary of PDX samples with NPM1c-Ab and NPM1-Ab.Table S5. RNAseq differential expression data of OCI-AML3-NPM1c-FKBP12 treated with dTag-13 for 3 or 24 h and OCI-AML3 cells treated with Selinexor for 6 h.Table S6. SLAMseq differential expression data of OCI-AML3-NPM1c-FKBP12 cells treated with dTag-13 for 15, 30, 60, 120 or 180 minutes.Table S7. PROseq differentially transcribed genes in OCI-AML3-NPM1c-FKBP12 cells treated with dTag-13 for 30 or 60 minutes and OCI-AML3 cells treated with VTP-50469 for 48 hours.Table S8. CRISPR tiling screen with NPM1 WT specific guides (blue) and NPM1c specific guides (red).Table S9. ChIPseq rpk values for CRM1 and HA ChIPs in 293T cells.Table S10. RNAseq differential gene expression analysis in 293T cells after overexpression of NPM1c-HA compared to all other samples (MIG, NPM1wt-HA and AS1-HA).Table S11. Fold change in ChIPseq differential peak interval signal in OCI-AML3 cells treated with Selinexor (100 nM, 6 hours) or VTP-50469 (330 nM, 96 hours).Table S12. Oligos used in this study.Table S13. Antibodies used in this study.Table S14. Summary of total and mapped reads of ChIPseq samples.补充表S1：未经处理与经dTAG-13处理的OCI-AML3-NPM1c-FKBP12细胞的染色质免疫共沉淀测序（ChIPseq）信号读段标准化比值； 补充表S2：NPM1c与野生型NPM1染色质结合靶基因总结； 补充表S3：本研究所用患者来源异种移植（PDX）模型的患者信息； 补充表S4：经NPM1c抗体（NPM1c-Ab）与NPM1抗体（NPM1-Ab）处理的PDX样本的ChIPseq每千碱基读段数（rpk）数据汇总； 补充表S5：经dTag-13处理3小时或24小时的OCI-AML3-NPM1c-FKBP12细胞，以及经Selinexor处理6小时的OCI-AML3细胞的RNA测序（RNAseq）差异表达数据； 补充表S6：经dTag-13处理15、30、60、120或180分钟的OCI-AML3-NPM1c-FKBP12细胞的瞬态转录组测序（SLAMseq）差异表达数据； 补充表S7：经dTag-13处理30分钟或60分钟的OCI-AML3-NPM1c-FKBP12细胞，以及经VTP-50469处理48小时的OCI-AML3细胞的精准转录组测序（PROseq）差异转录基因数据； 补充表S8：针对NPM1野生型（蓝色标记）与NPM1c（红色标记）的CRISPR平铺筛选向导RNA（guides）； 补充表S9：293T细胞中染色体区域维持蛋白1（CRM1）与血凝素标签（HA）的ChIPseq每千碱基读段数（rpk）值； 补充表S10：相较于其余对照样本（MIG空载对照、野生型NPM1-血凝素标签（NPM1wt-HA）与AS1-HA），过表达NPM1c-HA的293T细胞的RNAseq差异基因表达分析结果； 补充表S11：经100 nM Selinexor处理6小时，或经330 nM VTP-50469处理96小时的OCI-AML3细胞的ChIPseq差异峰区间信号倍数变化； 补充表S12：本研究所用寡核苷酸（Oligos）； 补充表S13：本研究所用抗体（Antibodies）； 补充表S14：ChIPseq样本的总读段与比对读段汇总