Imaging data for Dorison et al., 2022

Imaging data for the manuscript "Kidney organoids generated using an allelic series of NPHS2 point variants reveal distinct intracellular Podocin mistrafficking" dataset.zip (29.8GB uncompressed): Raw imaris (.ims) images taken using Andor Dragonfly Spinning Disk Confocal. Organised into subfolders by subcellular stain and then order of channels. Also contains a .txt file with metadata for every image. edited_ROIs.zip (8.46GB uncompressed): Podocyte region of interest for each image as TIFF files (.tiff), manually proofread by an expert human annotator (Aude Dorison). imageJ_rollingball.zip (32.4GB uncompressed): Relevant channels of each raw image with background subtraction (using rolling ball method with radius of 50 pixels) processed using Fiji/ImageJ as TIFF files (.tiff). This is used for downstream processing. Code is available at https://github.com/KidneyRegeneration/Dorison2022/本数据集对应论文《利用NPHS2点变异等位基因系列构建的肾类器官揭示Podocin蛋白（Podocin）的不同细胞内错误转运过程》，打包为dataset.zip，未压缩大小29.8GB：包含使用Andor Dragonfly转盘共聚焦显微镜采集的原始Imaris（.ims）格式图像，按亚细胞染色类型与通道顺序划分至多个子文件夹中。此外还附带一份记录所有图像元数据的文本文件（.txt）。 edited_ROIs.zip（未压缩大小8.46GB）：包含每张图像的足细胞（Podocyte）感兴趣区域（ROI）的TIFF（.tiff）格式文件，经专家人工标注员Aude Dorison手动校验修正。 imageJ_rollingball.zip（未压缩大小32.4GB）：包含经Fiji/ImageJ软件采用半径为50像素的滚球法完成背景扣除后的各原始图像对应通道的TIFF（.tiff）格式文件，用于后续下游处理。相关代码已开源至https://github.com/KidneyRegeneration/Dorison2022/