Monocyte/macrophage heterogeneity during skin wound healing in mice

Monocytes and macrophages isolated from skin wounds on male C57BL6/J mice on day 3, 6, and 10 post-injury, subjected to enzymatic digestion to obtain single cells and pooled from two mice per time point. Fresh cells with viability over 70% processed using the 10x Chromium platform. Libraries sequenced on HiSeq with paired-end reads. Fastq files were generated and demultiplexed into single cells using Cell Ranger software Single cell RNA-seq data from 10X Chromium. Three samples: WT_D3, WT_D6, and WT_D10