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<b>FIG 1 Nickel enhances HOSCN toxicity. </b><i>E. coli</i> strains MG1655 (wild-type) and MJG2314 (MG1655 ∆<i>corA761</i>::<i>kan</i>⁺) were grown in MOPS minimal medium without micronutrients containing 0.2% glucose, supplemented with 300 µM NiSO₄ and/or 400 µM HOSCN, as indicated, incubating at 37°C with shaking and measuring A₆₀₀ at 30-minute intervals for 24 hours (n=3 experimental replicates, with 3 technical replicates per experiment; error bars of 1 standard deviation).**图1 镍增强次硫氰酸（HOSCN）的毒性** 大肠杆菌（E. coli）菌株MG1655（野生型）与MJG2314（MG1655 ∆corA761::kan⁺）接种于不含微量营养元素的3-(N-吗啉代)丙磺酸（MOPS）基础培养基中，该培养基添加0.2%葡萄糖，并按实验设置添加300 μM硫酸镍（NiSO₄）和/或400 μM次硫氰酸（HOSCN）；于37℃条件下振荡培养，每隔30分钟测定一次600nm波长处的吸光度（A₆₀₀），持续培养24小时。本实验设置3次生物学重复，每次实验包含3次技术重复，误差棒代表1倍标准差。