Data on the antiviral and antioxidant activity of seven Thai medicinal plant extracts on the Porcine Reproductive and Respiratory Syndrome Virus

This dataset consists of four Excel <b>.xlsx</b> format spreadsheets. These are <b>virus titer_virus infection.xlsx</b>, <b>virus titer_replication.xlsx</b>, <b>phytochemical.xlsx </b>and <b>Cytotoxic.xlsx</b>.<br>Dataset <b>Cytotoxic.xlsx </b>shows data on the cytotoxic activity of the seven Thai medicinal plant extracts on the viability of MARC-145 cells. This was determined by the MTT assay. MARC-145 cells were incubated with various concentrations of the seven Thai medicinal plant extracts for 72 h prior to the MTT assay. Viability is shown as 50% cytotoxic concentrations (cc50). The cytotoxic concentration cc50 of the seven Thai medicinal plant extracts ranged from 78 ug/ml to 2,500 ug/ml. Average cytotoxic concentrations cc50 of each plant are shown in column 6, and standard deviation (SD) values are shown in column 7.<br>Dataset <b>phytochemical.xlsx </b>consists of 4 sheets labeled "Total phenol", "DPPH", "ABTS" and "Sheet 4". Sheet "Total phenol" shows the total phenol content of the of the seven Thai medicinal plant extracts. Total phenol content was calculated in micromoles (mM) gallic acid equivalents (GAE) per gram of plant extract (mM GAE/1g extract). Average and standard deviation values of total phenol content are shown in columns 6 and 7 respectively.Sheet "DPPH" shows data on the antioxidant activity of the 7 plant extracts. The free radical, 2, 2-Diphenyl-1- picrylhydrazyl (DPPH) is widely used to test the ability of compounds to act as free radical scavengers, and therefore to evaluate their antioxidant activity. Antioxidant activity was measured as the half maximal inhibitory concentration (IC50) of each extract in milligrams per milliliter (mg/ml). Average and standard deviation (SD) values are shown.Sheet "ABTS" shows data on the antioxidant activity of the 7 plant extracts. The 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay measures the relative ability of an antioxidant to scavenge the ABTS generated in aqueous phase. Antioxidant activity was measured as the half maximal inhibitory concentration (IC50) of each extract in milligrams per milliliter (mg/ml). Average and standard deviation (SD) values are shown.Sheet "FRAP" shows the results of the Ferric reducing antioxidant power (FRAP) assay using the 7 plant extracts. The assay uses antioxidants (in this case the plant extracts) as reductants in a redox-linked colorimetric reaction, wherein Fe³⁺ is reduced to Fe²⁺. Antioxidant activity was measured in millimolar concentration of Fe²⁺ per gram of plant extract (mM Fe²⁺/g). Average and standard deviation (SD) values are shown.<br>Dataset <b>virus titer_replication.xlsx </b>consists of 7 sheets labeled "CS", "GM", "HC", "CN", "PF", "PE" and "TT" and shows the virus titer in the inhibition of viral replication activity of the Thai medicinal plant extracts against PRRSV at 24, 48, and 72-h post-infection (hpi). The MARC-145 cellswere infected with PRRSV for 1 h and then placed in a medium containing the Thai medicinal plant extracts at various concentrations according to CC50 and incubated at24, 48, and 72 h, respectively. Viral titres were measured in Median Tissue Culture Infectious Dose (TCID50). TCID50 signifies the concentration at which 50% of the cells are infected. Each sheet shows data on the antiviral activity of each of the 7 plant extracts. <br>Dataset <b>virus titer_virus infection.xlsx </b>shows data on the virus titer of the inhibition of the viral infection activity of the seven Thai medicinal plant extracts against PRRSV at 24-h post infection (hpi). MARC-145 cells were infected with the Porcine reproductive and respiratory syndrome virus (PRRSV) at a multiplicity of infection of 1. Plant extracts were incubated with PRRSV for 1 h at 37°C before inoculation onto a monolayer of MARC-145 cells. Virus titres were quantified by the immunoperoxidase monolayer assay (IPMA). Virus titres were measured in TCID50.<b><br></b><b>Study aims and methodology:</b> The aim of this study was to determine the antiviral activities of seven Thai medicinal plant extracts against PRRSV infection <i>in vitro</i> and to measure their phytochemical contents to develop an alternative anti-PRRSV therapy for use in veterinary medicine.The Thai medicinal plants used in this study were Ethanolic extracts from the Thai medicinal plants <i>C. sappan</i> Linn., <i>G. mangostana </i>Linn., <i>H. cordata</i>, <i>P. frutescens</i>, <i>C. nutans</i>, <i>P. emblica</i>, and <i>T. triandra. </i>The cytotoxicity of the seven Thai medicinal plant extracts was determined on MARC-145 cells using the MTT cell viability assay. The following procedures are described in more detail in the published article: Inhibition of virus infection assay, Inhibition of virus replication assay, Determination of virus titer, phytochemical analysis and statistical analysis. <br>本数据集包含4个Excel格式（.xlsx）电子表格，分别为**virus titer_virus infection.xlsx**、**virus titer_replication.xlsx**、**phytochemical.xlsx**及**Cytotoxic.xlsx**。 **Cytotoxic.xlsx**展示了7种泰国药用植物提取物对MARC-145细胞活力的细胞毒活性数据，采用MTT比色法（MTT assay）测定。具体实验流程为：将MARC-145细胞与不同浓度的7种泰国药用植物提取物共孵育72小时后，进行MTT检测。细胞活力以50%细胞毒浓度（50% cytotoxic concentration, CC50）表示，7种提取物的CC50范围为78 μg/ml至2500 μg/ml。各植物的平均CC50值位于第6列，标准差（standard deviation, SD）值位于第7列。 **phytochemical.xlsx**包含4个工作表，分别命名为"Total phenol"、"DPPH"、"ABTS"及"Sheet 4"： 1. "Total phenol"工作表：展示7种泰国药用植物提取物的总酚含量，以每克提取物中没食子酸当量的微摩尔浓度（mM GAE/g extract，即每克提取物中相当于没食子酸的微摩尔数）计算。总酚含量的平均值及标准差分别位于第6列和第7列。 2. "DPPH"工作表：展示7种植物提取物的抗氧化活性数据。2,2-二苯基-1-苦肼基自由基（2,2-Diphenyl-1-picrylhydrazyl, DPPH）是常用的自由基清除能力检测底物，可用于评估化合物的抗氧化活性。本研究以半数抑制浓度（half maximal inhibitory concentration, IC50）作为抗氧化活性的评价指标，单位为mg/ml，各提取物的平均数值及标准差均已标注。 3. "ABTS"工作表：展示7种植物提取物的抗氧化活性数据。2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸)（2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS）检测法通过测定抗氧化剂清除水相生成的ABTS自由基的相对能力来评估抗氧化活性，同样以IC50（mg/ml）作为评价指标，包含平均值与标准差。 4. "FRAP"工作表：展示7种植物提取物的铁还原抗氧化能力（Ferric reducing antioxidant power, FRAP）检测结果。该检测以抗氧化剂（本研究中为植物提取物）作为还原剂，通过氧化还原关联的比色反应将Fe³+还原为Fe²+，以每克提取物中Fe²+的毫摩尔浓度（mM Fe²+/g）表示抗氧化活性，同时标注平均值与标准差。 **virus titer_replication.xlsx**包含7个工作表，分别为"CS"、"GM"、"HC"、"CN"、"PF"、"PE"及"TT"，展示了泰国药用植物提取物在感染后24、48及72小时（hours post-infection, hpi）时，对猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus, PRRSV）复制的抑制活性中的病毒滴度。实验操作如下：将MARC-145细胞用PRRSV感染1小时，随后更换含不同浓度泰国药用植物提取物的培养基（提取物浓度参照其CC50值），分别孵育24、48及72小时。病毒滴度以半数组织培养感染量（Median Tissue Culture Infectious Dose, TCID50）测定，TCID50指可使50%的细胞被感染的病毒浓度。每个工作表分别展示7种植物提取物各自的抗病毒活性数据。 **virus titer_virus infection.xlsx**展示了7种泰国药用植物提取物在感染后24小时（hpi）时，对PRRSV感染的抑制活性中的病毒滴度数据。实验中以感染复数为1的PRRSV感染MARC-145细胞；植物提取物先与PRRSV在37℃下共孵育1小时，再接种至MARC-145细胞单层。病毒滴度采用免疫过氧化物酶单层细胞试验（immunoperoxidase monolayer assay, IPMA）定量，以TCID50作为计量单位。 **研究目的与方法**：本研究旨在测定7种泰国药用植物提取物对PRRSV感染的体外（in vitro）抗病毒活性，并检测其植物化学成分含量，以期开发可用于兽医学的抗PRRSV替代疗法。本研究使用的泰国药用植物为7种植物的乙醇提取物，分别为*C. sappan* Linn.、*G. mangostana* Linn.、*H. cordata*、*P. frutescens*、*C. nutans*、*P. emblica*及*T. triandra*。本研究采用MTT细胞活力检测法测定7种泰国药用植物提取物对MARC-145细胞的细胞毒性。详细实验步骤已在已发表的文章中详述：包括病毒感染抑制试验、病毒复制抑制试验、病毒滴度测定、植物化学成分分析及统计学分析。