Raw data for degradation of dye

The application of microorganisms in azo dye remediation has gained significant attention, leading to various published studies reporting different methods for obtaining the best dye decolouriser. This paper investigates and compares the role of methods and media used in obtaining a bacterial consortium capable of decolourising azo dye as the sole carbon source, which is extremely rare to find. It was demonstrated that a prolonged acclimation under low substrate availability successfully isolated a novel consortium capable of utilising Reactive Red 120 dye as a sole carbon source in aerobic conditions. This consortium, known as JR3, consists of Pseudomonas aeruginosa strain MM01, Enterobacter sp. strain MM05 and Serratia marcescens strain MM06. Decolourised metabolites of consortium JR3 showed an improvement in mung bean’s seed germination and shoot and root length. One-factor-at-time optimisation characterisation showed maximal of 82.9% decolourisation at 0.7 g/L ammonium sulphate, pH 8, 35 °C, and RR120 concentrations of 200 ppm. Decolourisation modelling utilising response surface methodology (RSM) successfully improved decolourisation even more. RSM resulted in maximal decolourisation of 92.79% using 0.645 g/L ammonium sulphate, pH 8.29, 34.5 °C and 200 ppm RR120.微生物在偶氮染料（azo dye）修复中的应用已受到广泛关注，诸多已发表研究均报道了获取最优染料脱色菌群的各类方法。本研究针对以偶氮染料为唯一碳源的脱色细菌复合菌群——此类菌群极为罕见——的获取方法及培养基展开探究与对比分析。研究证实，在低底物供给条件下进行长期驯化，可成功分离得到一组可在有氧条件下以活性红120（Reactive Red 120）为唯一碳源的新型复合菌群。该复合菌群命名为JR3，由铜绿假单胞菌（Pseudomonas aeruginosa）菌株MM01、肠杆菌属（Enterobacter sp.）菌株MM05以及粘质沙雷氏菌（Serratia marcescens）菌株MM06组成。JR3复合菌群的脱色代谢产物可显著提升绿豆的种子萌发率以及幼苗株高与根长。单因素变量优化表征结果显示，在硫酸铵浓度0.7 g/L、pH值8、温度35℃、活性红120浓度200 ppm的条件下，脱色率可达82.9%。采用响应面法（RSM）进行脱色优化建模，可进一步提升脱色效率；经响应面法优化后，在硫酸铵浓度0.645 g/L、pH值8.29、温度34.5℃、活性红120浓度200 ppm的条件下，最高脱色率可达92.79%。