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scRNA-seq time course of cerebellar Nestin-expressing progenitors from neonatal mice with and without injury at birth

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To investigate the molecular changes that NEP subpopulations endure upon injury and to determine whether specific populations of NEPs have a transient increase in the signaling pathways associated with injury induced cellular stress and ROS, we performed multiplexed scRNA-seq of NEPs (CFP+) isolated by fluorescence activated cell sorting (FACS) from Nes-CFP/+ transgenic neonates with and without irradiation at timepoints P1, P2, P3 and P5.

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