Expression data of Tubb3+ cells from E4 chicken neural tube

The specification of the different neuronal subtypes is associated with a profound morphological transformation, involving processes common to most neurons and others characteristic of each neuronal subtype. To study the Tcf-dependent genes that were active during commissural neuron differentiation, we compared the genes in differentiating neurons (Tubb3+ cells) after the repression of Tcf-dependet transcription in chichen neural tube from E3 to E4, period when mostly commissural neurons are differentiating. We used Affymetrix GeneChip arrays to study TCF regulated genes during this process. We transfected E3 chick embryos for 24 h to produce either Tubb3::CAG:TcfEnR (suppression of Tcf-dependent transcription in neurons) or Tubb3::CAG:control (controls), dissected the NTs and cell-sorted the GFP+ neural populations. Both vectors are bicistronic and contain GFP CDS after IRES.