Supplementary Material for: FTO Genotype Interacts with Improvement in Aerobic Fitness on Body Weight Loss During Lifestyle Intervention

Objective: Not every participant responds with a comparable body weight loss to lifestyle intervention, despite the same compliance. Genetic factors may explain parts of this difference. Variation in fat mass and obesity-associated gene (FTO) is the strongest common genetic determinant of body weight. The aim of the present study was to evaluate the impact of FTO genotype differences in the link between improvement of fitness and reduction of body weight during a lifestyle intervention. Methods: We genotyped 292 healthy subjects for FTO rs8050136. Participants underwent a 9-month lifestyle intervention. Before and after intervention, aerobic fitness was tested by bicycle (VO2max) and treadmill spiroergometry (individual anaerobic threshold (IAT), subgroup of N = 192). Results: Participants lost body weight (p FTO genotype (p = 0.5). There was a significant correlation between improvement in VO2max and decrease in body weight (p FTO genotype interacted with this relationship (p = 0.0042 for VO2max, p = 0.0049 for IAT). When stratifying the cohort according to their improvement in VO2max, FTO obesity-risk A-allele carriers in the higher quartiles of improvement in fitness lost significantly less body weight. Conclusions: Our data reveal that genetic variation in FTO impacts on body weight reduction during lifestyle intervention only in subjects with marked improvement in aerobic fitness.研究目的：尽管所有受试者的干预依从性一致，但并非所有参与者对生活方式干预的体重减轻响应程度均相当。遗传因素或可解释该差异的部分成因。脂肪量与肥胖相关基因（fat mass and obesity-associated gene, FTO）的变异是目前已知最强的体重相关常见遗传决定因素。本研究旨在评估生活方式干预期间，FTO基因型差异对健身水平提升与体重减轻之间关联的影响。 方法：本研究对292名健康受试者进行了FTO rs8050136位点的基因分型。所有受试者均接受为期9个月的生活方式干预。干预前后，通过自行车测功仪检测有氧能力（VO2max，最大摄氧量），并通过跑台心肺运动试验检测个体无氧阈（IAT，该检测的亚组样本量N=192）。 结果：受试者体重出现显著下降（原文未标注对应P值）；不同FTO基因型受试者的体重变化无统计学差异（p=0.5）。VO2max提升与体重下降之间存在显著相关性（原文未标注对应P值）；且FTO基因型会对该关联产生交互作用（VO2max对应p=0.0042，IAT对应p=0.0049）。当根据VO2max提升程度对队列进行分层后，在健身提升程度较高的四分位组中，携带FTO肥胖风险A等位基因的受试者体重减轻量显著更少。 结论：本研究数据表明，仅在有氧能力提升显著的受试者中，FTO基因变异会影响生活方式干预期间的体重减轻效果。