MYCELIUM GROWTH OF PLEUROTUS SPP. IN RICE STRAW SUPPLEMENTED WITH LEATHER SAWDUST

ABSTRACT The objective of this study was to evaluate the biomass production, mycelial growth and colonization of rice-straw substrate by Pleurotus ostreatus and Pleurotus ostreatoroseus under the effect of adding vegetable-tanned leather sawdust. The first experiment consisted of the formulation of the culture media based on rice straw with the addition of leather sawdust at the concentrations of 0, 5, 10, 15, 20, 25 and 30%. Discs of mycelium of P. ostreatus (BF24) and P. ostreatoroseus (POR01/06) were transferred to the center of plates containing previously prepared media. They were incubated at 28° C. In the second experiment rice straw substrate underwent the same experimental conditions described above. The substrate inoculated with the two strains was placed in test tubes and incubated at 28° C. The fungal biomass was evaluated by the difference between wet weight and dry weight, while for the mycelial growth, measurements were performed at predetermined times until the complete colonization of the medium or substrate. For P. ostreatus, it was found that the treatments with the addition of 5, 15 and 25% and 15% of this supplement led to a lower biomass and mycelial growth, respectively. In the mycelial growth phase the addition of 5% of this supplement to the rice straw had a positive effect on the development of P. ostreatus.摘要 本研究旨在评估添加植物鞣制皮革锯末（vegetable-tanned leather sawdust）对糙皮侧耳（Pleurotus ostreatus）和桃红侧耳（Pleurotus ostreatoroseus）在稻草基质（rice-straw substrate）上的生物量产出、菌丝生长（mycelial growth）及基质定殖效果的影响。首个实验以稻草为基础配制培养基，分别添加0、5%、10%、15%、20%、25%及30%浓度的皮革锯末。将糙皮侧耳（BF24）与桃红侧耳（POR01/06）的菌丝菌饼转接至已制备好的培养基平板中央，于28℃条件下恒温培养。第二个实验采用相同的实验条件处理稻草基质。将接种了两株菌株的基质置于试管中，于28℃条件下恒温培养。采用湿重与干重的差值计算真菌生物量；针对菌丝生长情况，在预设时间节点开展测量，直至培养基或基质完全被菌丝定殖。针对糙皮侧耳，研究发现添加5%、15%、25%以及15%该添加剂的处理组，分别对应较低的生物量与菌丝生长水平；而在菌丝生长阶段，向稻草基质中添加5%的该添加剂可对糙皮侧耳的生长产生积极影响。