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Both the spindle microtubule-organizing centers and the nuclear pore complexes (NPCs) are convoluted structures where many signaling pathways converge to coordinate key events during cell division. Interestingly, despite their distinct molecular conformation and overall functions, these structures share common components and collaborate in the regulation of essential processes. We have established a new link between microtubule-organizing centers and nuclear pores in budding yeast by unveiling an interaction between the Bfa1/Bub2 complex, a mitotic exit inhibitor that localizes on the spindle pole bodies, and the Nup159 nucleoporin. Bfa1/Bub2 association with Nup159 is reduced in metaphase to not interfere with proper spindle positioning. However, their interaction is stimulated in anaphase and assists the Nup159-dependent autophagy pathway. The asymmetric localization of Bfa1/Bub2 during mitosis raises the possibility that its interaction with Nup159 could differentially promote Nup159-mediated autophagic processes, which might be relevant for the maintenance of the replicative lifespan.纺锤体微管组织中心（spindle microtubule-organizing centers）与核孔复合物（nuclear pore complexes，NPC）均为结构复杂的生物组装体，诸多信号通路在此汇聚，以协调细胞分裂过程中的关键事件。尽管二者分子构象与整体功能存在显著差异，却共享共有组分，并协同调控多项核心生命过程。本研究通过揭示定位于纺锤体极体的有丝分裂退出抑制剂Bfa1/Bub2复合物与Nup159核孔蛋白（Nup159 nucleoporin）之间的相互作用，在出芽酵母中建立了微管组织中心与核孔之间的全新关联。在细胞分裂中期，Bfa1/Bub2与Nup159的结合会被减弱，以避免干扰正常的纺锤体定位过程；而在分裂后期，二者的相互作用则被增强，进而辅助依赖于Nup159的自噬通路。有丝分裂过程中Bfa1/Bub2的不对称定位提示，其与Nup159的相互作用可能对Nup159介导的不同自噬过程产生差异化调控，这或许与细胞复制寿命的维持密切相关。