Data and statistical analysis for: Response to Elexacaftor/Tezacaftor/Ivacaftor in intestinal organoids derived from patients with cystic fibrosis

Data and code to reproduce all statistical analyses and figure in the manuscript "Response to Elexacaftor/Tezacaftor/Ivacaftor in intestinal organoids derived from patients with cystic fibrosis". The dataset is stored in the file `area_data.csv`, the columns are: `well` The ID of the well within the plate the measurement was taken `time` time from start of measurement `area` area of the organoids `patient` Anonymized ID of the patient `date` Date when the experiment was performed (date and patient ID identify the plate) `filename` The filename in raw data (not published) this plate was stored in. (uniquely identifies a plate) `mix` Identifies one od two variants of plate layouts used `replicate` ID of technical replicate (two technical replicates were done for each condition on the same plate) `type` The main condition - one of "TEZ/IVA", "ELX/TEZ/IVA", "FskOnly" (Control, only forskolin) `fsk_concentration` concentration of forskolin (μM) The file "organoids.Rmd" reproduces the analyses, other files are supporting to let the main analysis run. The analysis is written in R markdown.本数据集与配套代码可复现题为《囊性纤维化患者来源肠道类器官对依来卡托/泰扎卡托/依伐卡托的应答》的手稿中所有统计分析及图表制作。数据集存储于`area_data.csv`文件中，各列含义如下： - `well`：检测所在培养板的孔位编号 - `time`：检测开始后的计时时长 - `area`：类器官的面积 - `patient`：患者匿名编号 - `date`：实验开展日期（日期与患者编号可唯一标识一块培养板） - `filename`：该培养板对应的未公开原始数据的存储文件名，可唯一标识一块培养板 - `mix`：标识所用培养板布局的两种变体之一 - `replicate`：技术重复编号（同一块培养板上的每个实验条件均设置2次技术重复） - `type`：核心实验条件，可选值为"TEZ/IVA"、"ELX/TEZ/IVA"、"FskOnly"（仅添加福司柯林的对照组） - `fsk_concentration`：福司柯林的浓度，单位为μM 文件`organoids.Rmd`可复现本次分析流程，其余文件为辅助运行主分析的配套脚本，本次分析采用R Markdown撰写。