Phlorizin Prolongs the Lifespan of Caenorhabditis elegans by Insulin and SIR-2.1 Regulation

Phlorizin has significant antioxidant properties and was studied using Caenorhabditis elegans to explore potential antiaging mechanisms. Results showed that phlorizin mitigated the harmful effects of high temperatures and hydrogen peroxide, reduced oxidative stress, increased antioxidant enzyme activity, and reduced malondialdehyde levels. Network pharmacological analysis reveals that the AKT1, INSR, and SOD2 signaling pathways play a key role in the antiaging effects of phlorizin. Its action is mediated by insulin and SIR-2.1, influencing DAF-16, SKN-1, and downstream genes in the antiaging effects. This implicates phlorizin as a promising functional food additive targeting the DAF-16 and SOD-3 axes for antiaging.根皮苷（Phlorizin）具备显著的抗氧化特性，本研究以秀丽隐杆线虫（Caenorhabditis elegans）为实验模型，探究其潜在的抗衰老作用机制。实验结果显示，根皮苷可缓解高温与过氧化氢所引发的损伤，降低氧化应激水平，提升抗氧化酶活性，并降低丙二醛（malondialdehyde）含量。网络药理学分析表明，AKT1、INSR及SOD2信号通路在根皮苷的抗衰老作用中发挥关键调控作用。其抗衰老作用由胰岛素与SIR-2.1通路介导，可影响DAF-16、SKN-1及其下游基因的表达。上述研究结果证实，根皮苷是一款极具应用前景的抗衰老功能性食品添加剂，其作用靶点聚焦于DAF-16与SOD-3通路轴。