Comparison of combinatorial peptide library screening, proteome-derived library, and ProPeL motif discovery methodologies.

*Phosphorylatable residues (Ser and Thr) and Cys are not included in combinatorial peptide libraries used for kinase specificity determination.**In proteome-derived libraries motif width limits depend on whether the kinase reaction is performed before or after proteolytic peptide digestion.* 用于激酶特异性测定的组合肽库中，不包含可磷酸化氨基酸残基（丝氨酸Ser、苏氨酸Thr）以及半胱氨酸Cys。 * 在蛋白质组衍生肽库中，基序宽度限制取决于激酶反应是在肽段酶解前还是酶解后进行。