Poly I:C increases susceptibility to bacterial infection of the lung.
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A. Animals were administered i.n. poly I:C (50 µg), TLR7 agonist (imiquimod 50 µg or gardiquimod 50 µg) , or saline vehicle daily for 2 days. Twenty-four hours after the last dose, animals were given i.t. S. pneumoniae (900 CFU in 30 µl). On day 3, lungs were harvested for enumeration of CFU. *, pS. aureus (MRSA, LAC USA300; 6×106 CFU) 24 hours later. Lungs were harvested 24 hours after bacterial infection for enumeration of CFU. *, p
A. 将实验动物每日经鼻内（intranasal, i.n.）途径给予聚肌苷酸-聚胞苷酸（poly I:C，50 μg）、Toll样受体7激动剂（TLR7 agonist，咪喹莫特50 μg或加德莫德50 μg）或生理盐水赋形剂（saline vehicle），连续给药2天。末次给药后24小时，经气管内（intratracheal, i.t.）途径给予肺炎链球菌（Streptococcus pneumoniae, S. pneumoniae，30 μl溶媒中含900个菌落形成单位（CFU））。于第3天采集肺组织，进行菌落形成单位计数。*，p 24小时后，给予耐甲氧西林金黄色葡萄球菌（methicillin-resistant Staphylococcus aureus, MRSA，LAC USA300菌株，6×10^6 CFU），于细菌感染后24小时采集肺组织，进行菌落形成单位计数。*，p
